Produção de lipase por Bacillus licheniformis (UCP 1014) a partir de meios a base de resíduo da indústria de sorvetes

Abstract

The bacterium Bacillus licheniformis is a versatile, and has been used currently in production of various bitecnológicos. Lipases ( E.C.3.1.1.3) are enzymes found in nature and may be obtained from animal sources, plants and microbes, the latter being widely used due to several advantages presented against the other. The use of industrial wastes for production and formulation of means of production of various bioproducts, has emerged as an alternative to minimize the disposal of tailings from various manufacturing industries, especially those from food. Ice creams are food products obtained from an emulsion of fats and proteins, with or without the addition of other ingredients and substances which have been subjected to freezing their production generates large-scale solid-liquid waste with a large amount of fat and other organic wastes. In this work I study was conducted to select an alternative means for lipase production by B. licheniformis (UCP 1014). And then the formulation of an alternative means of production, using waste from ice cream industry through a factorial design, 24. The tests occurred during 96h at 37oC The results indicated that the middle called C (glucose 1.0%, peptone 2.0%, 0.5% yeast extract, olive oil, 1.0%, NaNO3 0.1%, 0.1% KH2PO4, MgSO4. 7H2O 0.05%), indicating a lipase activity of 256 (U / ml) / min. Tests of the factorial design, the test indicated that 11 had the highest activity of 480 U / mL for lipase production. The results suggest the reuse of waste oil from the ice cream industry for the development and production of microbial lipase.